src kinase inhibitor pp2 Search Results


src family kinase inhibitors pp1 and pp2  (Biomol GmbH)
90
Biomol GmbH src family kinase inhibitors pp1 and pp2
Src Family Kinase Inhibitors Pp1 And Pp2, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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src kinase inhibitor pp1  (Cayman Chemical)
90
Cayman Chemical src kinase inhibitor pp1
Src Kinase Inhibitor Pp1, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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src kinases inhibitor pp1  (Enzo Biochem)
90
Enzo Biochem src kinases inhibitor pp1
Src Kinases Inhibitor Pp1, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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src family tyrosine kinase inhibitor pp2  (Merck KGaA)
90
Merck KGaA src family tyrosine kinase inhibitor pp2
CD93 triggers the pY774-Cbl-dependent signaling pathway in spreading ECs. ( a ) Cell lysates from early spreading HUVECs pretreated for 30 min with vehicle alone (DMSO) or 10 μM <t>PP2</t> were analyzed by Western blotting using antibodies against pY774-Cbl and pY418-Src to confirm PP2 activity. Equal loading was confirmed by using anti-Cbl and anti-Src antibodies. ( b ) Quantification of pY774-Cbl phosphorylation levels from independent experiments performed as in ( a ). Values, normalized to Cbl protein levels, represent the percentage of pY774-Cbl levels relative to vehicle-treated cells (DMSO). *** p < 0.001; paired t -test. ( c , d ) HUVECs were transduced with lentiviral particles expressing unrelated (sh-unr) or CD93 shRNA (sh-CD93). Cells were detached from the plate, resuspended in complete growth medium, plated on the substrate, and fixed at early phases of spreading. Cells were analyzed by immunofluorescence using phalloidin, anti-CD93 antibody, and antibodies against pY774-Cbl ( c ) and Crk ( d ). White dot colocalization (wdc) images are shown as indicated. In the wdc pictures, magnification of the squared areas is shown (2.5×). Scale bars, 20 μm. ( e , f ) Quantification of cellular pY774-Cbl ( e ) and Crk ( f ) in the spreading border area (5 μm from the cell edge) of HUVECs transduced and treated as in ( c ) ( n = 14 cells for sh-unr and n = 16 cells for sh-CD93) and ( d ) ( n = 15 cells for sh-unr and n = 14 cells for sh-CD93). Data are presented as scatter plots and the fluorescence intensity of the pY774-Cbl +ve and Crk +ve signals per area is reported as arbitrary units (AU). * p < 0.05; Student t -test ( e ). *** p < 0.001; Mann–Whitney test ( f ).
Src Family Tyrosine Kinase Inhibitor Pp2, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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src family tyrosine kinase inhibitor pp2  (Biomol GmbH)
90
Biomol GmbH src family tyrosine kinase inhibitor pp2
CD93 triggers the pY774-Cbl-dependent signaling pathway in spreading ECs. ( a ) Cell lysates from early spreading HUVECs pretreated for 30 min with vehicle alone (DMSO) or 10 μM <t>PP2</t> were analyzed by Western blotting using antibodies against pY774-Cbl and pY418-Src to confirm PP2 activity. Equal loading was confirmed by using anti-Cbl and anti-Src antibodies. ( b ) Quantification of pY774-Cbl phosphorylation levels from independent experiments performed as in ( a ). Values, normalized to Cbl protein levels, represent the percentage of pY774-Cbl levels relative to vehicle-treated cells (DMSO). *** p < 0.001; paired t -test. ( c , d ) HUVECs were transduced with lentiviral particles expressing unrelated (sh-unr) or CD93 shRNA (sh-CD93). Cells were detached from the plate, resuspended in complete growth medium, plated on the substrate, and fixed at early phases of spreading. Cells were analyzed by immunofluorescence using phalloidin, anti-CD93 antibody, and antibodies against pY774-Cbl ( c ) and Crk ( d ). White dot colocalization (wdc) images are shown as indicated. In the wdc pictures, magnification of the squared areas is shown (2.5×). Scale bars, 20 μm. ( e , f ) Quantification of cellular pY774-Cbl ( e ) and Crk ( f ) in the spreading border area (5 μm from the cell edge) of HUVECs transduced and treated as in ( c ) ( n = 14 cells for sh-unr and n = 16 cells for sh-CD93) and ( d ) ( n = 15 cells for sh-unr and n = 14 cells for sh-CD93). Data are presented as scatter plots and the fluorescence intensity of the pY774-Cbl +ve and Crk +ve signals per area is reported as arbitrary units (AU). * p < 0.05; Student t -test ( e ). *** p < 0.001; Mann–Whitney test ( f ).
Src Family Tyrosine Kinase Inhibitor Pp2, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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inhibitor of src kinases 3-(4-chlorophenyl)-1-(1,1-dimethylethyl)-1h-pyrazolo[3,4-d]pyrimidin-4-amine pp2  (Cayman Chemical)
90
Cayman Chemical inhibitor of src kinases 3-(4-chlorophenyl)-1-(1,1-dimethylethyl)-1h-pyrazolo[3,4-d]pyrimidin-4-amine pp2
CD93 triggers the pY774-Cbl-dependent signaling pathway in spreading ECs. ( a ) Cell lysates from early spreading HUVECs pretreated for 30 min with vehicle alone (DMSO) or 10 μM <t>PP2</t> were analyzed by Western blotting using antibodies against pY774-Cbl and pY418-Src to confirm PP2 activity. Equal loading was confirmed by using anti-Cbl and anti-Src antibodies. ( b ) Quantification of pY774-Cbl phosphorylation levels from independent experiments performed as in ( a ). Values, normalized to Cbl protein levels, represent the percentage of pY774-Cbl levels relative to vehicle-treated cells (DMSO). *** p < 0.001; paired t -test. ( c , d ) HUVECs were transduced with lentiviral particles expressing unrelated (sh-unr) or CD93 shRNA (sh-CD93). Cells were detached from the plate, resuspended in complete growth medium, plated on the substrate, and fixed at early phases of spreading. Cells were analyzed by immunofluorescence using phalloidin, anti-CD93 antibody, and antibodies against pY774-Cbl ( c ) and Crk ( d ). White dot colocalization (wdc) images are shown as indicated. In the wdc pictures, magnification of the squared areas is shown (2.5×). Scale bars, 20 μm. ( e , f ) Quantification of cellular pY774-Cbl ( e ) and Crk ( f ) in the spreading border area (5 μm from the cell edge) of HUVECs transduced and treated as in ( c ) ( n = 14 cells for sh-unr and n = 16 cells for sh-CD93) and ( d ) ( n = 15 cells for sh-unr and n = 14 cells for sh-CD93). Data are presented as scatter plots and the fluorescence intensity of the pY774-Cbl +ve and Crk +ve signals per area is reported as arbitrary units (AU). * p < 0.05; Student t -test ( e ). *** p < 0.001; Mann–Whitney test ( f ).
Inhibitor Of Src Kinases 3 (4 Chlorophenyl) 1 (1,1 Dimethylethyl) 1h Pyrazolo[3,4 D]Pyrimidin 4 Amine Pp2, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inhibitor of src kinases 3-(4-chlorophenyl)-1-(1,1-dimethylethyl)-1h-pyrazolo[3,4-d]pyrimidin-4-amine pp2/product/Cayman Chemical
Average 90 stars, based on 1 article reviews
inhibitor of src kinases 3-(4-chlorophenyl)-1-(1,1-dimethylethyl)-1h-pyrazolo[3,4-d]pyrimidin-4-amine pp2 - by Bioz Stars, 2026-03
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src kinase inhibitor pp2  (Cosmo Bio USA)
90
Cosmo Bio USA src kinase inhibitor pp2
CD93 triggers the pY774-Cbl-dependent signaling pathway in spreading ECs. ( a ) Cell lysates from early spreading HUVECs pretreated for 30 min with vehicle alone (DMSO) or 10 μM <t>PP2</t> were analyzed by Western blotting using antibodies against pY774-Cbl and pY418-Src to confirm PP2 activity. Equal loading was confirmed by using anti-Cbl and anti-Src antibodies. ( b ) Quantification of pY774-Cbl phosphorylation levels from independent experiments performed as in ( a ). Values, normalized to Cbl protein levels, represent the percentage of pY774-Cbl levels relative to vehicle-treated cells (DMSO). *** p < 0.001; paired t -test. ( c , d ) HUVECs were transduced with lentiviral particles expressing unrelated (sh-unr) or CD93 shRNA (sh-CD93). Cells were detached from the plate, resuspended in complete growth medium, plated on the substrate, and fixed at early phases of spreading. Cells were analyzed by immunofluorescence using phalloidin, anti-CD93 antibody, and antibodies against pY774-Cbl ( c ) and Crk ( d ). White dot colocalization (wdc) images are shown as indicated. In the wdc pictures, magnification of the squared areas is shown (2.5×). Scale bars, 20 μm. ( e , f ) Quantification of cellular pY774-Cbl ( e ) and Crk ( f ) in the spreading border area (5 μm from the cell edge) of HUVECs transduced and treated as in ( c ) ( n = 14 cells for sh-unr and n = 16 cells for sh-CD93) and ( d ) ( n = 15 cells for sh-unr and n = 14 cells for sh-CD93). Data are presented as scatter plots and the fluorescence intensity of the pY774-Cbl +ve and Crk +ve signals per area is reported as arbitrary units (AU). * p < 0.05; Student t -test ( e ). *** p < 0.001; Mann–Whitney test ( f ).
Src Kinase Inhibitor Pp2, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/src kinase inhibitor pp2/product/Cosmo Bio USA
Average 90 stars, based on 1 article reviews
src kinase inhibitor pp2 - by Bioz Stars, 2026-03
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src family kinase inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (pp2)  (Biomol GmbH)
90
Biomol GmbH src family kinase inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (pp2)
CD93 triggers the pY774-Cbl-dependent signaling pathway in spreading ECs. ( a ) Cell lysates from early spreading HUVECs pretreated for 30 min with vehicle alone (DMSO) or 10 μM <t>PP2</t> were analyzed by Western blotting using antibodies against pY774-Cbl and pY418-Src to confirm PP2 activity. Equal loading was confirmed by using anti-Cbl and anti-Src antibodies. ( b ) Quantification of pY774-Cbl phosphorylation levels from independent experiments performed as in ( a ). Values, normalized to Cbl protein levels, represent the percentage of pY774-Cbl levels relative to vehicle-treated cells (DMSO). *** p < 0.001; paired t -test. ( c , d ) HUVECs were transduced with lentiviral particles expressing unrelated (sh-unr) or CD93 shRNA (sh-CD93). Cells were detached from the plate, resuspended in complete growth medium, plated on the substrate, and fixed at early phases of spreading. Cells were analyzed by immunofluorescence using phalloidin, anti-CD93 antibody, and antibodies against pY774-Cbl ( c ) and Crk ( d ). White dot colocalization (wdc) images are shown as indicated. In the wdc pictures, magnification of the squared areas is shown (2.5×). Scale bars, 20 μm. ( e , f ) Quantification of cellular pY774-Cbl ( e ) and Crk ( f ) in the spreading border area (5 μm from the cell edge) of HUVECs transduced and treated as in ( c ) ( n = 14 cells for sh-unr and n = 16 cells for sh-CD93) and ( d ) ( n = 15 cells for sh-unr and n = 14 cells for sh-CD93). Data are presented as scatter plots and the fluorescence intensity of the pY774-Cbl +ve and Crk +ve signals per area is reported as arbitrary units (AU). * p < 0.05; Student t -test ( e ). *** p < 0.001; Mann–Whitney test ( f ).
Src Family Kinase Inhibitor 4 Amino 5 (4 Chlorophenyl) 7 (T Butyl)Pyrazolo[3,4 D]Pyrimidine (Pp2), supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/src family kinase inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (pp2)/product/Biomol GmbH
Average 90 stars, based on 1 article reviews
src family kinase inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (pp2) - by Bioz Stars, 2026-03
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src kinase inhibitor pp2  (Oncogene Science Inc)
90
Oncogene Science Inc src kinase inhibitor pp2
CD93 triggers the pY774-Cbl-dependent signaling pathway in spreading ECs. ( a ) Cell lysates from early spreading HUVECs pretreated for 30 min with vehicle alone (DMSO) or 10 μM <t>PP2</t> were analyzed by Western blotting using antibodies against pY774-Cbl and pY418-Src to confirm PP2 activity. Equal loading was confirmed by using anti-Cbl and anti-Src antibodies. ( b ) Quantification of pY774-Cbl phosphorylation levels from independent experiments performed as in ( a ). Values, normalized to Cbl protein levels, represent the percentage of pY774-Cbl levels relative to vehicle-treated cells (DMSO). *** p < 0.001; paired t -test. ( c , d ) HUVECs were transduced with lentiviral particles expressing unrelated (sh-unr) or CD93 shRNA (sh-CD93). Cells were detached from the plate, resuspended in complete growth medium, plated on the substrate, and fixed at early phases of spreading. Cells were analyzed by immunofluorescence using phalloidin, anti-CD93 antibody, and antibodies against pY774-Cbl ( c ) and Crk ( d ). White dot colocalization (wdc) images are shown as indicated. In the wdc pictures, magnification of the squared areas is shown (2.5×). Scale bars, 20 μm. ( e , f ) Quantification of cellular pY774-Cbl ( e ) and Crk ( f ) in the spreading border area (5 μm from the cell edge) of HUVECs transduced and treated as in ( c ) ( n = 14 cells for sh-unr and n = 16 cells for sh-CD93) and ( d ) ( n = 15 cells for sh-unr and n = 14 cells for sh-CD93). Data are presented as scatter plots and the fluorescence intensity of the pY774-Cbl +ve and Crk +ve signals per area is reported as arbitrary units (AU). * p < 0.05; Student t -test ( e ). *** p < 0.001; Mann–Whitney test ( f ).
Src Kinase Inhibitor Pp2, supplied by Oncogene Science Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/src kinase inhibitor pp2/product/Oncogene Science Inc
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src kinase inhibitor pp2 - by Bioz Stars, 2026-03
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src-family kinase inhibitor pp2  (HFS LTD)
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HFS LTD src-family kinase inhibitor pp2
CD93 triggers the pY774-Cbl-dependent signaling pathway in spreading ECs. ( a ) Cell lysates from early spreading HUVECs pretreated for 30 min with vehicle alone (DMSO) or 10 μM <t>PP2</t> were analyzed by Western blotting using antibodies against pY774-Cbl and pY418-Src to confirm PP2 activity. Equal loading was confirmed by using anti-Cbl and anti-Src antibodies. ( b ) Quantification of pY774-Cbl phosphorylation levels from independent experiments performed as in ( a ). Values, normalized to Cbl protein levels, represent the percentage of pY774-Cbl levels relative to vehicle-treated cells (DMSO). *** p < 0.001; paired t -test. ( c , d ) HUVECs were transduced with lentiviral particles expressing unrelated (sh-unr) or CD93 shRNA (sh-CD93). Cells were detached from the plate, resuspended in complete growth medium, plated on the substrate, and fixed at early phases of spreading. Cells were analyzed by immunofluorescence using phalloidin, anti-CD93 antibody, and antibodies against pY774-Cbl ( c ) and Crk ( d ). White dot colocalization (wdc) images are shown as indicated. In the wdc pictures, magnification of the squared areas is shown (2.5×). Scale bars, 20 μm. ( e , f ) Quantification of cellular pY774-Cbl ( e ) and Crk ( f ) in the spreading border area (5 μm from the cell edge) of HUVECs transduced and treated as in ( c ) ( n = 14 cells for sh-unr and n = 16 cells for sh-CD93) and ( d ) ( n = 15 cells for sh-unr and n = 14 cells for sh-CD93). Data are presented as scatter plots and the fluorescence intensity of the pY774-Cbl +ve and Crk +ve signals per area is reported as arbitrary units (AU). * p < 0.05; Student t -test ( e ). *** p < 0.001; Mann–Whitney test ( f ).
Src Family Kinase Inhibitor Pp2, supplied by HFS LTD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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src kinase family inhibitor pp2  (Merck & Co)
90
Merck & Co src kinase family inhibitor pp2
CD93 triggers the pY774-Cbl-dependent signaling pathway in spreading ECs. ( a ) Cell lysates from early spreading HUVECs pretreated for 30 min with vehicle alone (DMSO) or 10 μM <t>PP2</t> were analyzed by Western blotting using antibodies against pY774-Cbl and pY418-Src to confirm PP2 activity. Equal loading was confirmed by using anti-Cbl and anti-Src antibodies. ( b ) Quantification of pY774-Cbl phosphorylation levels from independent experiments performed as in ( a ). Values, normalized to Cbl protein levels, represent the percentage of pY774-Cbl levels relative to vehicle-treated cells (DMSO). *** p < 0.001; paired t -test. ( c , d ) HUVECs were transduced with lentiviral particles expressing unrelated (sh-unr) or CD93 shRNA (sh-CD93). Cells were detached from the plate, resuspended in complete growth medium, plated on the substrate, and fixed at early phases of spreading. Cells were analyzed by immunofluorescence using phalloidin, anti-CD93 antibody, and antibodies against pY774-Cbl ( c ) and Crk ( d ). White dot colocalization (wdc) images are shown as indicated. In the wdc pictures, magnification of the squared areas is shown (2.5×). Scale bars, 20 μm. ( e , f ) Quantification of cellular pY774-Cbl ( e ) and Crk ( f ) in the spreading border area (5 μm from the cell edge) of HUVECs transduced and treated as in ( c ) ( n = 14 cells for sh-unr and n = 16 cells for sh-CD93) and ( d ) ( n = 15 cells for sh-unr and n = 14 cells for sh-CD93). Data are presented as scatter plots and the fluorescence intensity of the pY774-Cbl +ve and Crk +ve signals per area is reported as arbitrary units (AU). * p < 0.05; Student t -test ( e ). *** p < 0.001; Mann–Whitney test ( f ).
Src Kinase Family Inhibitor Pp2, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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src kinase family inhibitor pp2 - by Bioz Stars, 2026-03
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src-family tyrosine kinase inhibitor pp2  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc src-family tyrosine kinase inhibitor pp2
CD93 triggers the pY774-Cbl-dependent signaling pathway in spreading ECs. ( a ) Cell lysates from early spreading HUVECs pretreated for 30 min with vehicle alone (DMSO) or 10 μM <t>PP2</t> were analyzed by Western blotting using antibodies against pY774-Cbl and pY418-Src to confirm PP2 activity. Equal loading was confirmed by using anti-Cbl and anti-Src antibodies. ( b ) Quantification of pY774-Cbl phosphorylation levels from independent experiments performed as in ( a ). Values, normalized to Cbl protein levels, represent the percentage of pY774-Cbl levels relative to vehicle-treated cells (DMSO). *** p < 0.001; paired t -test. ( c , d ) HUVECs were transduced with lentiviral particles expressing unrelated (sh-unr) or CD93 shRNA (sh-CD93). Cells were detached from the plate, resuspended in complete growth medium, plated on the substrate, and fixed at early phases of spreading. Cells were analyzed by immunofluorescence using phalloidin, anti-CD93 antibody, and antibodies against pY774-Cbl ( c ) and Crk ( d ). White dot colocalization (wdc) images are shown as indicated. In the wdc pictures, magnification of the squared areas is shown (2.5×). Scale bars, 20 μm. ( e , f ) Quantification of cellular pY774-Cbl ( e ) and Crk ( f ) in the spreading border area (5 μm from the cell edge) of HUVECs transduced and treated as in ( c ) ( n = 14 cells for sh-unr and n = 16 cells for sh-CD93) and ( d ) ( n = 15 cells for sh-unr and n = 14 cells for sh-CD93). Data are presented as scatter plots and the fluorescence intensity of the pY774-Cbl +ve and Crk +ve signals per area is reported as arbitrary units (AU). * p < 0.05; Student t -test ( e ). *** p < 0.001; Mann–Whitney test ( f ).
Src Family Tyrosine Kinase Inhibitor Pp2, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


CD93 triggers the pY774-Cbl-dependent signaling pathway in spreading ECs. ( a ) Cell lysates from early spreading HUVECs pretreated for 30 min with vehicle alone (DMSO) or 10 μM PP2 were analyzed by Western blotting using antibodies against pY774-Cbl and pY418-Src to confirm PP2 activity. Equal loading was confirmed by using anti-Cbl and anti-Src antibodies. ( b ) Quantification of pY774-Cbl phosphorylation levels from independent experiments performed as in ( a ). Values, normalized to Cbl protein levels, represent the percentage of pY774-Cbl levels relative to vehicle-treated cells (DMSO). *** p < 0.001; paired t -test. ( c , d ) HUVECs were transduced with lentiviral particles expressing unrelated (sh-unr) or CD93 shRNA (sh-CD93). Cells were detached from the plate, resuspended in complete growth medium, plated on the substrate, and fixed at early phases of spreading. Cells were analyzed by immunofluorescence using phalloidin, anti-CD93 antibody, and antibodies against pY774-Cbl ( c ) and Crk ( d ). White dot colocalization (wdc) images are shown as indicated. In the wdc pictures, magnification of the squared areas is shown (2.5×). Scale bars, 20 μm. ( e , f ) Quantification of cellular pY774-Cbl ( e ) and Crk ( f ) in the spreading border area (5 μm from the cell edge) of HUVECs transduced and treated as in ( c ) ( n = 14 cells for sh-unr and n = 16 cells for sh-CD93) and ( d ) ( n = 15 cells for sh-unr and n = 14 cells for sh-CD93). Data are presented as scatter plots and the fluorescence intensity of the pY774-Cbl +ve and Crk +ve signals per area is reported as arbitrary units (AU). * p < 0.05; Student t -test ( e ). *** p < 0.001; Mann–Whitney test ( f ).

Journal: International Journal of Molecular Sciences

Article Title: CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells

doi: 10.3390/ijms222212417

Figure Lengend Snippet: CD93 triggers the pY774-Cbl-dependent signaling pathway in spreading ECs. ( a ) Cell lysates from early spreading HUVECs pretreated for 30 min with vehicle alone (DMSO) or 10 μM PP2 were analyzed by Western blotting using antibodies against pY774-Cbl and pY418-Src to confirm PP2 activity. Equal loading was confirmed by using anti-Cbl and anti-Src antibodies. ( b ) Quantification of pY774-Cbl phosphorylation levels from independent experiments performed as in ( a ). Values, normalized to Cbl protein levels, represent the percentage of pY774-Cbl levels relative to vehicle-treated cells (DMSO). *** p < 0.001; paired t -test. ( c , d ) HUVECs were transduced with lentiviral particles expressing unrelated (sh-unr) or CD93 shRNA (sh-CD93). Cells were detached from the plate, resuspended in complete growth medium, plated on the substrate, and fixed at early phases of spreading. Cells were analyzed by immunofluorescence using phalloidin, anti-CD93 antibody, and antibodies against pY774-Cbl ( c ) and Crk ( d ). White dot colocalization (wdc) images are shown as indicated. In the wdc pictures, magnification of the squared areas is shown (2.5×). Scale bars, 20 μm. ( e , f ) Quantification of cellular pY774-Cbl ( e ) and Crk ( f ) in the spreading border area (5 μm from the cell edge) of HUVECs transduced and treated as in ( c ) ( n = 14 cells for sh-unr and n = 16 cells for sh-CD93) and ( d ) ( n = 15 cells for sh-unr and n = 14 cells for sh-CD93). Data are presented as scatter plots and the fluorescence intensity of the pY774-Cbl +ve and Crk +ve signals per area is reported as arbitrary units (AU). * p < 0.05; Student t -test ( e ). *** p < 0.001; Mann–Whitney test ( f ).

Article Snippet: The Src family tyrosine kinase inhibitor PP2 was purchased from Merck KGaA.

Techniques: Western Blot, Activity Assay, Phospho-proteomics, Transduction, Expressing, shRNA, Immunofluorescence, Fluorescence, MANN-WHITNEY